EEA1 Antibody (2G2)
Novus Biologicals, part of Bio-Techne | Catalog # H00008411-M03
Key Product Details
Species Reactivity
Human, Mouse, Hamster
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2G2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
EEA1 (NP_003557, 1312 a.a. ~ 1411 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG
Reactivity Notes
Hamster reactivity reported in scientific literature (PMID: 25419208).
Specificity
EEA1 - early endosome antigen 1, 162kD (2G2)
Marker
Early Endosome Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for EEA1 Antibody (2G2)
![Western Blot: EEA1 Antibody (2G2) [H00008411-M03]](https://resources.bio-techne.com/images/products/EEA1-Antibody-2G2-Western-Blot-H00008411-M03-img0004.jpg "Western Blot: EEA1 Antibody (2G2) [H00008411-M03]")
Western Blot: EEA1 Antibody (2G2) [H00008411-M03]
Western Blot: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of EEA1 expression in A-431 (Cat # L015V1).![Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03]](https://resources.bio-techne.com/images/products/EEA1-Antibody-2G2-Immunocytochemistry-Immunofluorescence-H00008411-M03-img0003.jpg "Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03]")
Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03]
Immunocytochemistry/Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of monoclonal antibody to EEA1 on HeLa cell. Antibody concentration 10 ug/ml![ELISA: EEA1 Antibody (2G2) [H00008411-M03]](https://resources.bio-techne.com/images/products/EEA1-Antibody-2G2-ELISA-H00008411-M03-img0002.jpg "ELISA: EEA1 Antibody (2G2) [H00008411-M03]")
ELISA: EEA1 Antibody (2G2) [H00008411-M03]
ELISA: EEA1 Antibody (2G2) [H00008411-M03] - Detection limit for recombinant GST tagged EEA1 is approximately 0.03ng/ml as a capture antibody.Applications for EEA1 Antibody (2G2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EEA1
Long Name
Early Endosome Antigen 1
Alternate Names
MST105, MSTP105, ZFYVE2
Entrez Gene IDs
8411 (Human)
Gene Symbol
EEA1
UniProt
Additional EEA1 Products
Product Documents for EEA1 Antibody (2G2)
Product Specific Notices for EEA1 Antibody (2G2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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