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EED Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38907

Novus Biologicals, part of Bio-Techne
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NBP2-38907
NBP2-38907-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EED Antibody

Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907]

Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907]

Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining in human tonsil and pancreas tissues.. Corresponding EED RNA-seq data are presented for the same tissues.
Western Blot: EED Antibody [NBP2-38907]

Western Blot: EED Antibody [NBP2-38907]

Western Blot: EED Antibody [NBP2-38907] - Analysis using Anti-EED antibody NBP2-38907 (A) shows similar pattern to independent antibody NBP2-57195 (B).
Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907]

Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907]

Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Applications for EED Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EED

EED encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Embryonic Ectoderm Development

Alternate Names

WAIT1

Gene Symbol

EED

UniProt

Additional EED Products

Product Documents for EED Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EED Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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