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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

EED antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EED. The immunogen is located within amino acids 30 - 80 of EED. Amino Acid Squence: ESGTNTERPDTPTNTPNA

Specificity

Four alternatively spliced transcript variants have been observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EED Antibody - BSA Free

Western Blot: EED AntibodyBSA Free [NBP2-81819]

Western Blot: EED AntibodyBSA Free [NBP2-81819]

Western Blot: EED Antibody [NBP2-81819] - Western blot analysis of EED expression in human testis tissue lysate with EED antibody at 2 ug/ml
Immunocytochemistry/ Immunofluorescence: EED Antibody - BSA Free [NBP2-81819]

Immunocytochemistry/ Immunofluorescence: EED Antibody - BSA Free [NBP2-81819]

Immunocytochemistry/Immunofluorescence: EED Antibody [NBP2-81819] - Immunofluorescence of EED in human heart tissue with EED antibody at 20 ug/mL.
Western Blot: EED AntibodyBSA Free [NBP2-81819]

Western Blot: EED AntibodyBSA Free [NBP2-81819]

Western Blot: EED Antibody [NBP2-81819] - Analysis of EED in human heart tissue lysate with EED antibody at (A) 1 and (B) 2 ug/ml.

Applications for EED Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EED

EED encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Embryonic Ectoderm Development

Alternate Names

WAIT1

Gene Symbol

EED

Additional EED Products

Product Documents for EED Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EED Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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