eEF-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-87020

Novus Biologicals, part of Bio-Techne
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NB100-87020
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all eEF-2 Antibodies »

Product Summary for eEF-2 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 808 and 858 of human eukaryotic translation elongation factor 2 using the numbering given in entry NP_001952.1 (GeneID 1938).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eEF-2 Antibody

Western Blot: eEF-2 Antibody [NB100-87020]

Western Blot: eEF-2 Antibody [NB100-87020]

Western Blot: eEF-2 Antibody [NB100-87020] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.
Immunohistochemistry-Paraffin: eEF-2 Antibody [NB100-87020]

Immunohistochemistry-Paraffin: eEF-2 Antibody [NB100-87020]

Immunohistochemistry-Paraffin: eEF-2 Antibody [NB100-87020] - Section of human breast carcinoma (left) and mouse teratoma (right).Antibody: Affinity purified rabbit anti-eEF2 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Applications for eEF-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:2000-1:10000
Application Notes
This antibody is not recommended for immunoprecipitation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: eEF-2

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq]

Long Name

Eukaryotic Translation Elongation Factor 2

Alternate Names

eEF2

Entrez Gene IDs

1938 (Human)

Gene Symbol

EEF2

UniProt

NP_001952.1 (Human)

Additional eEF-2 Products

Product Documents for eEF-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eEF-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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