eEF1A1 Antibody (0X0H8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15399
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0X0H8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for eEF1A1 Antibody (0X0H8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human eEF1A1 (NP_001393.1). LQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for eEF1A1 Antibody (0X0H8)
![Western Blot: eEF1A1 Antibody (0X0H8) [NBP3-15399]](https://resources.bio-techne.com/images/products/eEF1A1-Antibody-0X0H8-Western-Blot-NBP3-15399-img0004.jpg "Western Blot: eEF1A1 Antibody (0X0H8) [NBP3-15399]")
Western Blot: eEF1A1 Antibody (0X0H8) [NBP3-15399]
Western Blot: eEF1A1 Antibody (0X0H8) [NBP3-15399] - Western blot analysis of extracts of various cell lines, using eEF1A1 Rabbit mAb (NBP3-15399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: eEF1A1 Antibody (0X0H8) [NBP3-15399] -
Immunocytochemistry/Immunofluorescence: eEF1A1 Antibody (0X0H8) [NBP3-15399] -Confocal imaging of C6 cells using eEF1A1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.Applications for eEF1A1 Antibody (0X0H8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: eEF1A1
Alternate Names
CCS3, CCS-3, cervical cancer suppressor 3, CTCL tumor antigen, EE1A1, EEF-1, EEF1A, eEF1A-1, EF1AHNGC:16303, EF1a-like protein, EF-1-alpha-1, EF-Tu, elongation factor 1 alpha subunit, elongation factor 1-alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, eukaryotic translation elongation factor 1 alpha 1, eukaryotic translation elongation factor 1 alpha 1-like 14, FLJ25721, glucocorticoid receptor AF-1 specific elongation factor, GRAF-1EF, LENG7, leukocyte receptor cluster (LRC) member 7, Leukocyte receptor cluster member 7, MGC102687, MGC131894, MGC16224, prostate tumor-inducing protein 1, PTI1, translation elongation factor 1 alpha 1-like 14
Gene Symbol
EEF1A1
Additional eEF1A1 Products
Product Documents for eEF1A1 Antibody (0X0H8)
Product Specific Notices for eEF1A1 Antibody (0X0H8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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