EEF1A2 [p Ser358] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29510

Novus Biologicals, part of Bio-Techne
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NBP2-29510
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Primate (100%), Rat (100%), Sheep (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EEF1A2 Antibodies »

Product Summary for EEF1A2 [p Ser358] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 conjugated to KLH. Accession # Q05639

Modification

p Ser358

Specificity

Specific for endogenous levels of the ~50 kDa eEF1A2 phosphorylated at Ser358. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEF1A2 [p Ser358] Antibody

Western Blot: EEF1A2 [p Ser358] Antibody [NBP2-29510]

Western Blot: EEF1A2 [p Ser358] Antibody [NBP2-29510]

Western Blot: EEF1A2 [p Ser358] Antibody [NBP2-29510] - Western blot of mouse hippocampus lysate showing specific immunolabeling of the ~ 50k eEF1A2 protein phosphorylated at Ser358. Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).

Applications for EEF1A2 [p Ser358] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EEF1A2

Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013).

Alternate Names

eEF1A-2, EEF1ALFLJ41696, EF1A, EF-1-alpha-2, elongation factor 1-alpha 2, elongation factor-1 alpha, Eukaryotic elongation factor 1 A-2, eukaryotic translation elongation factor 1 alpha 2, HS1, statin, statin S1, statin-like, statin-S1, STN, STNL

Entrez Gene IDs

1917 (Human)

Gene Symbol

EEF1A2

UniProt

Q05639 (Human)

Additional EEF1A2 Products

Product Documents for EEF1A2 [p Ser358] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EEF1A2 [p Ser358] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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