EEF1E1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89186

Novus Biologicals, part of Bio-Techne
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NBP1-89186
NBP1-89186-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EEF1E1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF1E1 Antibody

Western Blot: EEF1E1 Antibody [NBP1-89186]

Western Blot: EEF1E1 Antibody [NBP1-89186]

Western Blot: EEF1E1 Antibody [NBP1-89186] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-89186]

Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-89186]

Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-89186] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: EEF1E1 Antibody [NBP1-89186]

Western Blot: EEF1E1 Antibody [NBP1-89186]

Western Blot: EEF1E1 Antibody [NBP1-89186] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
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Applications for EEF1E1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1E1

EEF1E1 encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

AIMP3Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, ARS-interacting multifunctional protein 3, Elongation factor p18, eukaryotic translation elongation factor 1 epsilon 1, eukaryotic translation elongation factor 1 epsilon-1, Multisynthase complex auxiliary component p18, P18, p18 component of aminoacyl-tRNA synthetase complex

Gene Symbol

EEF1E1

Additional EEF1E1 Products

Product Documents for EEF1E1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EEF1E1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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