EEFSEC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83992

Novus Biologicals, part of Bio-Techne
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NBP1-83992
NBP1-83992-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EEFSEC Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEFSEC Antibody

Western Blot: EEFSEC Antibody [NBP1-83992]

Western Blot: EEFSEC Antibody [NBP1-83992]

Western Blot: EEFSEC Antibody [NBP1-83992] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-173
Immunocytochemistry/ Immunofluorescence: EEFSEC Antibody [NBP1-83992]

Immunocytochemistry/ Immunofluorescence: EEFSEC Antibody [NBP1-83992]

Immunocytochemistry/Immunofluorescence: EEFSEC Antibody [NBP1-83992] - Staining of human cell line A-431 shows localization to nucleus, nuclear bodies & cytosol.
Immunohistochemistry-Paraffin: EEFSEC Antibody [NBP1-83992]

Immunohistochemistry-Paraffin: EEFSEC Antibody [NBP1-83992]

Immunohistochemistry-Paraffin: EEFSEC Antibody [NBP1-83992] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.

Applications for EEFSEC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEFSEC

Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replacesEF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP

Alternate Names

eukaryotic elongation factor, selenocysteine-tRNA-specific

Gene Symbol

EEFSEC

Additional EEFSEC Products

Product Documents for EEFSEC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EEFSEC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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