EEN Antibody (1B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00006455-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EEN Antibodies »

Product Specifications

Immunogen

SH3GL1 (AAH01270, 1 a.a. ~ 368 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSVAGLKKQFYKASQLVSEKVGGAEGTKLDDDFKEMEKKVDVTSKAVTEVLARTIEYLQPNPASRAKLTMLNTVSKIRGQVKNPGYPQSEGLLGECMIRHGKELGGESNFGDALLDAGESMKRLAEVKDSLDIEVKQNFIDPLQNLCEKDLKEIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFEESKEVAETSMHNLLETDIEQVSQLSALVDAQLDYHRQAVQILDELAEKLKRRMREASSRPKREYKPKPREPFDLGEPEQSNGGFPCTTAPKIAASSSFRSSDKPIRTPSRSMPPLDQPSCKALYDFEPENDGELGFHEGDVITLTNQIDENWYEGMLDGQSGFFPLSYVEVLVPLPQ

Specificity

SH3GL1 - SH3-domain GRB2-like 1 (1B8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EEN Antibody (1B8)

Western Blot: EEN Antibody (1B8) [H00006455-M05]

Western Blot: EEN Antibody (1B8) [H00006455-M05]

Western Blot: EEN Antibody (1B8) [H00006455-M05] - Analysis of SH3GL1 expression in HeLa.
Sandwich ELISA: EEN Antibody (1B8) [H00006455-M05] - Detection limit for recombinant GST tagged SH3GL1 is 0.3 ng/ml as a capture antibody.

Applications for EEN Antibody (1B8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EEN

Endophilin A2 is an SH3 domain containing protein implicated in endocytosis. It has been identified in human acute leukemia as a fusion partner of MLL (mixed-lineage leukemia). Because of its involvement in this chromosomal translocation, endophilin A2 is also known as EEN (extra eleven nineteen).

Alternate Names

CNSA1endophilin-2, EEN fusion partner of MLL, EENSH3 domain-containing GRB2-like protein 1, Endophilin-2, extra 11-19 leukemia fusion, Extra eleven-nineteen leukemia fusion gene protein, MGC111371, SH3 domain protein 2B, SH3-containing Grb-2-like 1 protein, SH3D2Bendophilin-A2, SH3-domain GRB2-like 1, SH3P8

Entrez Gene IDs

6455 (Human)

Gene Symbol

SH3GL1

UniProt

AAH01270 (Human)

Additional EEN Products

Product Documents for EEN Antibody (1B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EEN Antibody (1B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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