EEN Antibody (GT3065)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13631
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT3065
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for EEN Antibody (GT3065)
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse EEN. The exact sequence is proprietary.
Reactivity Notes
Human (85%), Bovine (83%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for EEN Antibody (GT3065)
![Western Blot: EEN Antibody (GT3065) [NBP3-13631]](https://resources.bio-techne.com/images/products/EEN-Antibody-GT3065-Western-Blot-NBP3-13631-img0003.jpg "Western Blot: EEN Antibody (GT3065) [NBP3-13631]")
Western Blot: EEN Antibody (GT3065) [NBP3-13631]
Western Blot: EEN Antibody (GT3065) [NBP3-13631] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EEN antibody [GT3065] (NBP3-13631) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: EEN Antibody (GT3065) [NBP3-13631]](https://resources.bio-techne.com/images/products/EEN-Antibody-GT3065-Immunocytochemistry-Immunofluorescence-NBP3-13631-img0001.jpg "Immunocytochemistry/ Immunofluorescence: EEN Antibody (GT3065) [NBP3-13631]")
Immunocytochemistry/ Immunofluorescence: EEN Antibody (GT3065) [NBP3-13631]
Immunocytochemistry/Immunofluorescence: EEN Antibody (GT3065) [NBP3-13631] - EEN antibody [GT3065] detects EEN protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEN stained by EEN antibody [GT3065] (NBP3-13631) diluted at 1:500. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: EEN Antibody (GT3065) [NBP3-13631]](https://resources.bio-techne.com/images/products/EEN-Antibody-GT3065-Immunohistochemistry-Paraffin-NBP3-13631-img0002.jpg "Immunohistochemistry-Paraffin: EEN Antibody (GT3065) [NBP3-13631]")
Immunohistochemistry-Paraffin: EEN Antibody (GT3065) [NBP3-13631]
Immunohistochemistry-Paraffin: EEN Antibody (GT3065) [NBP3-13631] - EEN antibody [GT3065] detects EEN protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. EEN stained by EEN antibody [GT3065] (NBP3-13631) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for EEN Antibody (GT3065)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EEN
Alternate Names
CNSA1endophilin-2, EEN fusion partner of MLL, EENSH3 domain-containing GRB2-like protein 1, Endophilin-2, extra 11-19 leukemia fusion, Extra eleven-nineteen leukemia fusion gene protein, MGC111371, SH3 domain protein 2B, SH3-containing Grb-2-like 1 protein, SH3D2Bendophilin-A2, SH3-domain GRB2-like 1, SH3P8
Gene Symbol
SH3GL1
Additional EEN Products
Product Documents for EEN Antibody (GT3065)
Product Specific Notices for EEN Antibody (GT3065)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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