EFHC1 Antibody (4E7)
Novus Biologicals, part of Bio-Techne | Catalog # H00114327-M09
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
EFHC1 (NP_060570.1, 270 a.a. ~ 378 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DDTVEIREVHERNDGRDPFPLLMNRQRVPKVLVENAKNFPQCVLEISDQEVLEWYTAKDFIVGKSLTILGRTFFIYDCDPFTRRYYKEKFGITDLPRIDVSKREPPPVK
Specificity
Reacts with EF-hand domain (C-terminal) containing 1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for EFHC1 Antibody (4E7)
![Western Blot: EFHC1 Antibody (4E7) [H00114327-M09]](https://resources.bio-techne.com/images/products/EFHC1-Antibody-4E7-Western-Blot-H00114327-M09-img0002.jpg "Western Blot: EFHC1 Antibody (4E7) [H00114327-M09]")
Western Blot: EFHC1 Antibody (4E7) [H00114327-M09]
Western Blot: EFHC1 Antibody (4E7) [H00114327-M09] - Analysis of EFHC1 expression in transfected 293T cell line by EFHC1 monoclonal antibody (M09), clone 4E7. Lane 1: EFHC1 transfected lysate (Predicted MW: 73.9 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: EFHC1 Antibody (4E7) [H00114327-M09] - Detection limit for recombinant GST tagged EFHC1 is 0.3 ng/ml as a capture antibody.
Applications for EFHC1 Antibody (4E7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EFHC1
Alternate Names
dJ304B14.2, EF-hand domain (C-terminal) containing 1, EF-hand domain-containing protein 1, EJA1, EJM1, FLJ10466, FLJ37290, JAE, myoclonin-1
Entrez Gene IDs
114327 (Human)
Gene Symbol
EFHC1
UniProt
Additional EFHC1 Products
Product Documents for EFHC1 Antibody (4E7)
Product Specific Notices for EFHC1 Antibody (4E7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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