EFHD2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1477
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CAAFKELQSTFK corresponding to C-Terminus according to NP_077305.2.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23959172).
Specificity
This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EFHD2 Antibody
Western Blot: EFHD2 Antibody [NB100-1477]
Western Blot: EFHD2 Antibody [NB100-1477] - Staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477]
Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477] - Analysis of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for EFHD2 Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 4 ug/ml
Immunohistochemistry-Paraffin
2 - 4 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
0.3 - 10 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human tonsil, thymus and lymph node and in mouse thymus and lymph node lysates (calculated MW of 26.7 kDa band according to NP_077305.2). IHC-P: Human tonsil shows membranous staining in selected cells.
Reviewed Applications
Read 1 review rated 5 using NB100-1477 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: EFHD2
Alternate Names
EF hand domain containing 2, EF hand domain family, member D2, EF-hand domain family, member D2, EF-hand domain-containing protein D2, MGC4342, swiprosin 1, swiprosin-1, SWS1
Gene Symbol
EFHD2
UniProt
Additional EFHD2 Products
Product Documents for EFHD2 Antibody
Product Specific Notices for EFHD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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