EFTUD2 Antibody (1-D10-A1-R)

Western Blot: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295]

Western Blot: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Western blot analysis of EFTUD2 on different lysates with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: LNCaP cell lysate
Lane 6: HepG2 cell lysate
Lane 7: K-562 cell lysate
Lane 8: C6 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: RAW264.7 cell lysate
Lane 12: Mouse testis tissue lysate
Lane 13: Mouse kidney tissue lysate
Lane 14: Rat brain tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 109 kDa
Observed band size: 120 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32295) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295]

Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling EFTUD2 with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32295, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295]

Immunohistochemistry: EFTUD2 Antibody (1-D10-A1-R) [NBP3-32295] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EFTUD2 antibody (NBP3-32295) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32295) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.