Eg5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-181
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Drosophila, Mammal, Plant
Cited:
Human, Mouse, Porcine, Insect - Drosophila, Plant
Applications
Validated:
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A recombinant segment of the coiled-coil domain of human Eg5
Reactivity Notes
Predicted to react with most mammalian species. Drosophila reactivity reported in scientific literature (PMID: 23888285). Mouse reactivity reported in scientific literature (PMID: 27117404). Plant reactivity reported in scientific literature (PMID: 19527496).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Eg5 Antibody
![Western Blot: Eg5 Antibody [NB500-181]](https://resources.bio-techne.com/images/products/Eg5-Antibody-Western-Blot-NB500-181-img0005.jpg "Western Blot: Eg5 Antibody [NB500-181]")
Western Blot: Eg5 Antibody [NB500-181]
Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.![Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181]](https://resources.bio-techne.com/images/products/Eg5-Antibody-Immunocytochemistry-Immunofluorescence-NB500-181-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181]")
Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181]
Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.![Western Blot: Eg5 Antibody [NB500-181]](https://resources.bio-techne.com/images/products/Eg5-Antibody-Western-Blot-NB500-181-img0006.jpg "Western Blot: Eg5 Antibody [NB500-181]")
Western Blot: Eg5 Antibody [NB500-181]
Western Blot: Eg5 Antibody [NB500-181] - Human Eg5 rescues depletion of the Drosophila kinesin-5 family member Klp61F. Western blot analysis of cell lysates prepared from induced and uninduced; control and Klp61F RNAi-treated cells blotted with anti-Eg5 to monitor induction of Eg5-mCherry, anti-Klp61F to confirm depletion of the motor and anti-alpha-tubulin as a loading control. Cells depleted of Klp61F and with Eg5-mCherry induced are referred to as RepEg5 cells. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fonc.2013.00187/abstract), licensed under a CC-BY license.Applications for Eg5 Antibody
Application
Recommended Usage
Immunoblotting
reported in scientific literature (PMID 28392145)
Immunocytochemistry/ Immunofluorescence
1:1000
Immunoprecipitation
1:10-1:500
Western Blot
1:1000
Application Notes
A band at ~125 kDa can be detected by Western blot.
Reviewed Applications
Read 2 reviews rated 2.5 using NB500-181 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Eg5
Long Name
Kinesin-like protein KIF11
Alternate Names
KIF11, KNSL1, TRIP-5, TRIP5
Gene Symbol
KIF11
UniProt
Additional Eg5 Products
Product Documents for Eg5 Antibody
Product Specific Notices for Eg5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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