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EGF Antibody (RM0078-2M13) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21708

Novus Biologicals, part of Bio-Techne
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NBP1-21708
NBP1-21708-0.025mg

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0078-2M13

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for EGF Antibody (RM0078-2M13) - Azide and BSA Free

Immunogen

Mouse recombinant EGF

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for EGF Antibody (RM0078-2M13) - Azide and BSA Free

Immunohistochemistry-Paraffin: EGF Antibody (RM0078-2M13) - Azide and BSA Free [NBP1-21708]

Immunohistochemistry-Paraffin: EGF Antibody (RM0078-2M13) - Azide and BSA Free [NBP1-21708]

Immunohistochemistry-Paraffin: EGF Antibody (RM0078-2M13) [NBP1-21708] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP1-21708

Applications for EGF Antibody (RM0078-2M13) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EGF

Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Entrez Gene IDs

1950 (Human)

Gene Symbol

EGF

Additional EGF Products

Product Documents for EGF Antibody (RM0078-2M13) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EGF Antibody (RM0078-2M13) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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