EGLN2/PHD1 Antibody (2G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00112398-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EGLN2/PHD1 Antibodies »

Product Specifications

Immunogen

EGLN2 (AAH36051, 1 a.a. ~ 407 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDSPCQPQPLSQALPQLPGSSSEPLEPEPGRARMGVESYLPCPLLPSYHCPGVPSEASAGSGTPRATATSTTASPLRDGFGGQDGGELRPLQSEGAAALVTKGCQRLAAQGARPEAPKRKWAEDGGDAPSPSKRPWARQENQEAEREGGMSCSCSSGSGEASAGLMEEALPSAPERLALDYIVPCMRYYGICVKDSFLGAALGGRVLAEVEALKRGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGSYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAAKDKYQLASGQKGVQVPVSQPPTPT

Specificity

EGLN2 - egl nine homolog 2 (C. elegans) (2G3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for EGLN2/PHD1 Antibody (2G3)

Western Blot: EGLN2/PHD1 Antibody (2G3) [H00112398-M01]

Western Blot: EGLN2/PHD1 Antibody (2G3) [H00112398-M01]

Western Blot: EGLN2/PHD1 Antibody (2G3) [H00112398-M01] - Western Blot detection against Immunogen (70.51 KDa) .
Sandwich ELISA: EGLN2/PHD1 Antibody (2G3) [H00112398-M01] - Detection limit for recombinant GST tagged EGLN2 is 0.3 ng/ml as a capture antibody.

Applications for EGLN2/PHD1 Antibody (2G3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EGLN2/PHD1

The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this posttranslational modification. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Long Name

Egl Nine Homolog 2/Prolyl Hydroxylase Domain-containing Protein 1

Alternate Names

EIT6, HIFPH1, HPH-1, HPH-3, PHD1

Entrez Gene IDs

112398 (Human)

Gene Symbol

EGLN2

UniProt

AAH36051 (Human)

Additional EGLN2/PHD1 Products

Product Documents for EGLN2/PHD1 Antibody (2G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EGLN2/PHD1 Antibody (2G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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