EGLN2/PHD1 Antibody (2G9M7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16265

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16265-100ul
NBP3-16265-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2G9M7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EGLN2/PHD1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EGLN2/PHD1 (Q96KS0). MDSPCQPQPLSQALPQLPGSSSEPLEPEPGRARMGVESYLPCPLLPSYHCPGVPSEASAGSGTPRATATSTTASPLRDGFGGQDGGELRPLQSEGAAALV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EGLN2/PHD1 Antibody (2G9M7)

Western Blot: EGLN2/PHD1 Antibody (2G9M7) [NBP3-16265]

Western Blot: EGLN2/PHD1 Antibody (2G9M7) [NBP3-16265]

Western Blot: EGLN2/PHD1 Antibody (2G9M7) [NBP3-16265] - Analysis of extracts of various cell lines, using EGLN2/PHD1 Rabbit mAb (NBP3-16265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for EGLN2/PHD1 Antibody (2G9M7)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EGLN2/PHD1

HIF prolyl hydroxylase 1 (HPH-3 or EGLN2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.

Long Name

Egl Nine Homolog 2/Prolyl Hydroxylase Domain-containing Protein 1

Alternate Names

EIT6, HIFPH1, HPH-1, HPH-3, PHD1

Gene Symbol

EGLN2

Additional EGLN2/PHD1 Products

Product Documents for EGLN2/PHD1 Antibody (2G9M7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EGLN2/PHD1 Antibody (2G9M7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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