EGLN2/PHD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76551

Novus Biologicals, part of Bio-Techne
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NBP2-76551
NBP2-76551-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EGLN2/PHD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLSQALPQLPGSSSEPLEPEPGRARMGVESYLPCPLLPSYHCPGVPSEASAGSGTPRATATSTTASPLRDGFGGQDGGE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EGLN2/PHD1 Antibody

Immunocytochemistry/ Immunofluorescence: EGLN2/PHD1 Antibody [NBP2-76551]

Immunocytochemistry/ Immunofluorescence: EGLN2/PHD1 Antibody [NBP2-76551]

Immunocytochemistry/Immunofluorescence: EGLN2/PHD1 Antibody [NBP2-76551] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.

Applications for EGLN2/PHD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGLN2/PHD1

HIF prolyl hydroxylase 1 (HPH-3 or EGLN2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.

Long Name

Egl Nine Homolog 2/Prolyl Hydroxylase Domain-containing Protein 1

Alternate Names

EIT6, HIFPH1, HPH-1, HPH-3, PHD1

Entrez Gene IDs

112398 (Human)

Gene Symbol

EGLN2

UniProt

Q96KS0 (Human)

Additional EGLN2/PHD1 Products

Product Documents for EGLN2/PHD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EGLN2/PHD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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