EGLN2/PHD1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05472

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all EGLN2/PHD1 Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide made to an N-terminal portion of the human EGLN2 / PHD1 protein (between amino acids 10-75) [UniProt Q96KS0]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EGLN2/PHD1 Antibody - BSA Free

Western Blot: EGLN2/PHD1 AntibodyBSA Free [NBP3-05472]

Western Blot: EGLN2/PHD1 AntibodyBSA Free [NBP3-05472]

Western Blot: EGLN2/PHD1 Antibody [NBP3-05472] - Total protein from human HeLa, K562, HepG2 and U87 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-EGLN2/PHD1 (NBP2-59179) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
Immunocytochemistry/ Immunofluorescence: EGLN2/PHD1 Antibody - BSA Free [NBP3-05472]

Immunocytochemistry/ Immunofluorescence: EGLN2/PHD1 Antibody - BSA Free [NBP3-05472]

Immunocytochemistry/Immunofluorescence: EGLN2/PHD1 Antibody [NBP3-05472] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-EGLN2/PHD1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for EGLN2/PHD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2 - 5 ug/ml

Western Blot

1 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGLN2/PHD1

HIF prolyl hydroxylase 1 (HPH-3 or EGLN2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.

Long Name

Egl Nine Homolog 2/Prolyl Hydroxylase Domain-containing Protein 1

Alternate Names

EIT6, HIFPH1, HPH-1, HPH-3, PHD1

Gene Symbol

EGLN2

Additional EGLN2/PHD1 Products

Product Documents for EGLN2/PHD1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EGLN2/PHD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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