EGLN3/PHD3 Antibody (EG188e/d5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30440

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat, Ovine

Predicted:

Bovine (98%), Mouse (98%), Primate (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EG188e/d5

Format

BSA Free

Concentration

1 mg/ml

View all EGLN3/PHD3 Antibodies »

Product Specifications

Immunogen

Partial recombinant human PHD3/HIF Prolyl Hydroxylase 3 (UniProt# Q9H6Z9)

Localization

Cytoplasm. Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EGLN3/PHD3 Antibody (EG188e/d5) - BSA Free

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)BSA Free [NBP1-30440]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)BSA Free [NBP1-30440]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - PHD3 expression in SK-N-BE(2) whole cell lysates. Cells were treated in normoxia (left) and in hypoxia (right). Image from verified customer review.
Immunohistochemistry: EGLN3/PHD3 Antibody (EG188e/d5) - BSA Free [NBP1-30440]

Immunohistochemistry: EGLN3/PHD3 Antibody (EG188e/d5) - BSA Free [NBP1-30440]

Immunohistochemistry: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - Analysis of HIF Prolyl Hydroxylase 3 in human renal cancer using DAB with hematoxylin counterstain.
Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)BSA Free [NBP1-30440]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)BSA Free [NBP1-30440]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - Analysis of HIF Prolyl Hydroxylase 3 in (1) Cobalt chloride treated COS-7 nuclear extracts and (2) Untreated COS-7 nuclear extracts.
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Applications for EGLN3/PHD3 Antibody (EG188e/d5) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Simple Western

1:1000

Western Blot

10 ug/ml
Application Notes
This HIF Prolyl Hydroxylase 3 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen approx. 27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 4 reviews rated 4.8 using NBP1-30440 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGLN3/PHD3

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) which are known for their role in mediating physiological responses to hypoxic stress via modulation of HIF1alpha expression levels. HIF-alpha subunit is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). When oxygen levels are normal, HIF Prolyl Hydroxylase 3 (HIF-PH3/ PHD3/ Egln3) hydroxylates a specific proline found in HIF1A'a NODD/CODD domains and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. Once hydroxylated, HIFs undergo proteasomal degradation via von Hippel-Lindau (VHL) ubiquitination complex. Upon hypoxic trigger, the hydroxylation reaction is tempered which let HIFs to escape degradation process leading to their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Besides HIFs, in hypoxic conditions HIF-PH3 also hydroxylates PKM (thereby limiting glycolysis) and hydroxylates/regulates the stability of ADRB2. In cardiomyocytes, HIF-PH3 inhibits Bcl2's anti-apoptotic effect by disrupting the BAX-BCL2 complex, and in neurons, HIF-PH3 has a NGF-induced proapoptotic effect mediated via CASP3 activity regulation. HIF-PH3 also plays a crucial role in DNA damage response by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway.

Long Name

Egl Nine Homolog 3/Prolyl Hydroxylase Domain-containing Protein 3

Alternate Names

HIFPH3, PHD3

Entrez Gene IDs

112399 (Human)

Gene Symbol

EGLN3

UniProt

Q9H6Z9

Additional EGLN3/PHD3 Products

Product Documents for EGLN3/PHD3 Antibody (EG188e/d5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EGLN3/PHD3 Antibody (EG188e/d5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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