EGLN3/PHD3 Antibody (JE60-82)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32745
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE60-82
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full length protein within human EGLN3/PHD3. (Uniprot: Q9H6Z9)
Localization
Cytoplasm. Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EGLN3/PHD3 Antibody (JE60-82)
Western Blot: EGLN3/PHD3 Antibody (JE60-82) [NBP3-32745]
Western Blot: EGLN3/PHD3 Antibody (JE60-82) [NBP3-32745] - Western blot analysis of EGLN3/PHD3 on different lysates with Rabbit anti-EGLN3/PHD3 antibody (NBP3-32745) at 1/1,000 dilution.Lane 1: Mouse liver tissue lysate (40 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: A375 cell lysate (20 ug/Lane)
Lane 4: HEK-293 cell lysate (20 ug/Lane)
Predicted band size: 27 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for EGLN3/PHD3 Antibody (JE60-82)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EGLN3/PHD3
Long Name
Egl Nine Homolog 3/Prolyl Hydroxylase Domain-containing Protein 3
Alternate Names
HIFPH3, PHD3
Gene Symbol
EGLN3
Additional EGLN3/PHD3 Products
Product Documents for EGLN3/PHD3 Antibody (JE60-82)
Product Specific Notices for EGLN3/PHD3 Antibody (JE60-82)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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