EGLN3/PHD3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-303

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Chemotaxis, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all EGLN3/PHD3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23732909). Rat reactivity reported in scientific literature (PMID: 23545307).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EGLN3/PHD3 Antibody - BSA Free

Western Blot: EGLN3/PHD3 AntibodyBSA Free [NB100-303]

Western Blot: EGLN3/PHD3 AntibodyBSA Free [NB100-303]

Western Blot: EGLN3/PHD3 Antibody [NB100-303] - Whole cell protein from MEF cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/mL anti-EGLN3/PHD3 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Simple Western: EGLN3/PHD3 AntibodyBSA Free [NB100-303]

Simple Western: EGLN3/PHD3 AntibodyBSA Free [NB100-303]

Simple Western: EGLN3/PHD3 Antibody [NB100-303] - Image shows a specific band for PHD3/HIF Prolyl Hydroxylase 3 in 0.5 mg/mL of Hypoxic HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody - BSA Free [NB100-303]

Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody - BSA Free [NB100-303]

Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-303] - LDL Receptor was detected in immersion fixed paraffin-embedded sections of human liver cancer using rabbit anti-human antibody (NB100-303) at 1:3000 overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent (VC003) with DAB chromogen (brown) and counterstained with hematoxylin (blue).
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Applications for EGLN3/PHD3 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 21620138)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 20801873)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 25161887)

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 15721254)

Simple Western

1:1000

Western Blot

1:500-1:2000
Application Notes
In Western blot, a band is seen at approx. 27 kDa. This antibody has been tested against HeLa and MEF cell lysates. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NB100-303 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGLN3/PHD3

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) which are known for their role in mediating physiological responses to hypoxic stress via modulation of HIF1alpha expression levels. HIF-alpha subunit is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). When oxygen levels are normal, HIF Prolyl Hydroxylase 3 (HIF-PH3/ PHD3/ Egln3) hydroxylates a specific proline found in HIF1A'a NODD/CODD domains and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. Once hydroxylated, HIFs undergo proteasomal degradation via von Hippel-Lindau (VHL) ubiquitination complex. Upon hypoxic trigger, the hydroxylation reaction is tempered which let HIFs to escape degradation process leading to their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Besides HIFs, in hypoxic conditions HIF-PH3 also hydroxylates PKM (thereby limiting glycolysis) and hydroxylates/regulates the stability of ADRB2. In cardiomyocytes, HIF-PH3 inhibits Bcl2's anti-apoptotic effect by disrupting the BAX-BCL2 complex, and in neurons, HIF-PH3 has a NGF-induced proapoptotic effect mediated via CASP3 activity regulation. HIF-PH3 also plays a crucial role in DNA damage response by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway.

Long Name

Egl Nine Homolog 3/Prolyl Hydroxylase Domain-containing Protein 3

Alternate Names

HIFPH3, PHD3

Gene Symbol

EGLN3

Additional EGLN3/PHD3 Products

Product Documents for EGLN3/PHD3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EGLN3/PHD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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