EGR1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78775
![Western Blot: EGR1 Antibody [NBP1-78775]](https://resources.bio-techne.com/images/products/EGR1-Antibody-Western-Blot-NBP1-78775-img0002.jpg "Western Blot: EGR1 Antibody [NBP1-78775]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Cited:
Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of mouse Early Growth Response Protein 1 using the numbering given in entry NP_031939.1 (GeneID 13653).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EGR1 Antibody
Western Blot: EGR1 Antibody [NBP1-78775]
Western Blot: EGR1 Antibody [NBP1-78775] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), NIH3T3 (M; 50 mcg), human erythroleukemia (E; 50 mcg), and Jurkat (J; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-EGR1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EGR1 was also immunoprecipitated by rabbit anti-EGR1 antibody NBP1-78776 which recognizes a disparate epitope.Applications for EGR1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:1000-1:5000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30846562). Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EGR1
Long Name
Early Growth Response 1
Alternate Names
KROX24, NGFIA, ZNF225
Gene Symbol
EGR1
UniProt
Additional EGR1 Products
Product Documents for EGR1 Antibody
Product Specific Notices for EGR1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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