EGR2 Antibody (27C3)

Western Blot: EGR2 Antibody (27C3) [NBP3-26605] -

Western Blot: EGR2 Antibody (27C3) [NBP3-26605] - Positive Western Blot detected in: SY5Y whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate.
All lanes: EGR2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 50 kDa
Observed band size: 50 kDa

Flow Cytometry: EGR2 Antibody (27C3) [NBP3-26605] -

Flow Cytometry: EGR2 Antibody (27C3) [NBP3-26605] - Overlay Peak curve showing SH-SY5Y cells stained with EGR2 Antibody (27C3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.