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EGR2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16286

Novus Biologicals, part of Bio-Techne
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NBP2-16286

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Rat

Predicted:

Porcine (95%), Rat (90%), Rhesus Macaque (98%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EGR2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EGR2 Antibody

Western Blot: EGR2 Antibody [NBP2-16286]

Western Blot: EGR2 Antibody [NBP2-16286]

Western Blot: EGR2 Antibody [NBP2-16286] - Expression in transfected 293T cell line. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286]

Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286]

Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using antibody at 1:500 dilution.
EGR2 Antibody

Western Blot: EGR2 Antibody [NBP2-16286] -

Western Blot: EGR2 Antibody [NBP2-16286] - Sample (30 ug of whole cell lysate)
A: zebrafish brain
10% SDS PAGE
NBP2-16286 diluted at 1:1000

Applications for EGR2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:35151688) .

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EGR2

The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

AT591, CMT1D, CMT4E, early growth response 2, early growth response 2 (Krox-20 homolog, Drosophila), early growth response protein 2, EGR-2, FLJ14547, Krox-20 homolog, Drosophila, KROX-20, Drosophila, homolog (early growth response-2), KROX20DKFZp686J1957, Zinc finger protein Krox-20

Gene Symbol

EGR2

UniProt

Additional EGR2 Products

Product Documents for EGR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EGR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.