EHBP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93614
![Western Blot: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Western-Blot-NBP1-93614-img0006.jpg "Western Blot: EHBP1 Antibody [NBP1-93614]")
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MRSAKSASSSEELINKLNFLDEAEKDLATVNSNPFDDPDAAELNPFGDPDSEEPITETASPRKTEDSFYNNSYNPFKEVQTPQYLNPFDEPEAFV
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EHBP1 Antibody
![Western Blot: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Western-Blot-NBP1-93614-img0014.jpg "Western Blot: EHBP1 Antibody [NBP1-93614]")
Western Blot: EHBP1 Antibody [NBP1-93614]
Western Blot: EHBP1 Antibody [NBP1-93614] - EHD2 localizes to the plasma membrane independent of its interaction partners, syndapin2 and EHBP1.HeLa cells were treated with non-targeting control-, syndapin2- or EHBP1-siRNA for 72 h. Cells were then lysed, and equal amounts of proteins were separated by SDS-PAGE and subjected to immunoblotting with antibodies against syndapin2, EHBP1, EHD2, caveolin1 or actin. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0074519), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-93614-img0004.jpg "Immunocytochemistry/ Immunofluorescence: EHBP1 Antibody [NBP1-93614]")
Immunocytochemistry/ Immunofluorescence: EHBP1 Antibody [NBP1-93614]
Immunocytochemistry/Immunofluorescence: EHBP1 Antibody [NBP1-93614] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-93614-img0013.jpg "Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]")
Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]
Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614] - Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.![Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-93614-img0008.jpg "Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]")
![Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-93614-img0009.jpg "Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]")
![Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-93614-img0011.jpg "Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]")
![Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]](https://resources.bio-techne.com/images/products/EHBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-93614-img0012.jpg "Immunohistochemistry-Paraffin: EHBP1 Antibody [NBP1-93614]")
Applications for EHBP1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:20-1:50
Western Blot
1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EHBP1
Alternate Names
EH domain binding protein 1
Gene Symbol
EHBP1
Additional EHBP1 Products
Product Documents for EHBP1 Antibody
Product Specific Notices for EHBP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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