EHD1 Antibody (5S4P9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16201
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5S4P9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EHD1 (Q9H4M9). MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EHD1 Antibody (5S4P9)
![Western Blot: EHD1 Antibody (5S4P9) [NBP3-16201]](https://resources.bio-techne.com/images/products/EHD1-Antibody-5S4P9-Western-Blot-NBP3-16201-img0002.jpg "Western Blot: EHD1 Antibody (5S4P9) [NBP3-16201]")
Western Blot: EHD1 Antibody (5S4P9) [NBP3-16201]
Western Blot: EHD1 Antibody (5S4P9) [NBP3-16201] - Western blot analysis of extracts of various cell lines, using EHD1 Rabbit mAb (NBP3-16201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.![Immunohistochemistry-Paraffin: EHD1 Antibody (5S4P9) [NBP3-16201]](https://resources.bio-techne.com/images/products/EHD1-Antibody-5S4P9-Immunohistochemistry-Paraffin-NBP3-16201-img0001.jpg "Immunohistochemistry-Paraffin: EHD1 Antibody (5S4P9) [NBP3-16201]")
Immunohistochemistry-Paraffin: EHD1 Antibody (5S4P9) [NBP3-16201]
Immunohistochemistry-Paraffin: EHD1 Antibody (5S4P9) [NBP3-16201] - Immunohistochemistry of paraffin-embedded human appendix using EHD1 Rabbit mAb (NBP3-16201) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for EHD1 Antibody (5S4P9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: EHD1
Alternate Names
EH-domain containing 1
Gene Symbol
EHD1
Additional EHD1 Products
Product Documents for EHD1 Antibody (5S4P9)
Product Specific Notices for EHD1 Antibody (5S4P9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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