EHD3 Antibody (4B7)

Novus Biologicals, part of Bio-Techne | Catalog # H00030845-M01

Novus Biologicals, part of Bio-Techne
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H00030845-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EHD3 Antibodies »

Product Specifications

Immunogen

EHD3 (NP_055415, 357 a.a. ~ 406 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPI

Reactivity Notes

Other species not tested.

Specificity

EHD3 - EH-domain containing 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EHD3 Antibody (4B7)

Western Blot: EHD3 Antibody (4B7) [H00030845-M01]

Western Blot: EHD3 Antibody (4B7) [H00030845-M01]

Western Blot: EHD3 Antibody (4B7) [H00030845-M01] - EHD3 monoclonal antibody (M01), clone 4B7 Analysis of EHD3 expression in IMR-32.
Immunocytochemistry/ Immunofluorescence: EHD3 Antibody (4B7) [H00030845-M01]

Immunocytochemistry/ Immunofluorescence: EHD3 Antibody (4B7) [H00030845-M01]

Immunocytochemistry/Immunofluorescence: EHD3 Antibody (4B7) [H00030845-M01] - Analysis of monoclonal antibody to EHD3 on HeLa cell. Antibody concentration 25 ug/ml.
Immunohistochemistry-Paraffin: EHD3 Antibody (4B7) [H00030845-M01]

Immunohistochemistry-Paraffin: EHD3 Antibody (4B7) [H00030845-M01]

Immunohistochemistry-Paraffin: EHD3 Antibody (4B7) [H00030845-M01] - Analysis of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
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Applications for EHD3 Antibody (4B7)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EHD3

The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus.

Alternate Names

EH domain containing 3, EH domain-containing protein 3, EHD2, EH-domain containing 3, PAST homolog 3, PAST3

Entrez Gene IDs

30845 (Human)

Gene Symbol

EHD3

OMIM

605891 (Human)

UniProt

NP_055415 (Human)

Additional EHD3 Products

Product Documents for EHD3 Antibody (4B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EHD3 Antibody (4B7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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