EHD4 Antibody (1B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00030844-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EHD4 Antibodies »

Product Specifications

Immunogen

EHD4 (AAH06287, 1 a.a. ~ 541 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFSWMGRQAGGRERAGGADAVQTVTGGLRSLYLRKVLPLEEAYRFHEFHSPALEDADFENKPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISVIDSPGILSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQDLFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPSVFGKENKKRELISRLPEIYIQLQREYQISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLPPHLVPPSHRKSLPKAD

Localization

Endoplasmic reticulum and Nuclear

Specificity

EHD4 - EH-domain containing 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EHD4 Antibody (1B8)

Western Blot: EHD4 Antibody (1B8) [H00030844-M01]

Western Blot: EHD4 Antibody (1B8) [H00030844-M01]

Western Blot: EHD4 Antibody (1B8) [H00030844-M01] - EHD4 monoclonal antibody (M01), clone 1B8 Analysis of EHD4 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: EHD4 Antibody (1B8) [H00030844-M01]

Immunocytochemistry/ Immunofluorescence: EHD4 Antibody (1B8) [H00030844-M01]

Immunocytochemistry/Immunofluorescence: EHD4 Antibody (1B8) [H00030844-M01] - Analysis of monoclonal antibody to EHD4 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: EHD4 Antibody (1B8) [H00030844-M01]

Western Blot: EHD4 Antibody (1B8) [H00030844-M01]

Western Blot: EHD4 Antibody (1B8) [H00030844-M01] - Analysis of EHD4 expression in transfected 293T cell line by EHD4 monoclonal antibody (M01), clone 1B8.Lane 1: EHD4 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.

Applications for EHD4 Antibody (1B8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, tissue lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EHD4

EHD4 is an EH domain containing protein expressed in the extracellular matrix. EHD4 is the first extracellular matrix protein described that contains an EH domain.

Alternate Names

EH domain containing 4, EH domain-containing protein 4, EH-domain containing 4, HCA10, HCA11, Hepatocellular carcinoma-associated protein 10/11, hepatocellular carcinoma-associated protein HCA11, ortholog of rat pincher, PAST homolog 4, PAST4

Entrez Gene IDs

30844 (Human)

Gene Symbol

EHD4

OMIM

605892 (Human)

UniProt

AAH06287 (Human)

Additional EHD4 Products

Product Documents for EHD4 Antibody (1B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EHD4 Antibody (1B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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