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EHD4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16287

Novus Biologicals, part of Bio-Techne
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NBP2-16287

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for EHD4 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human EHD4. The exact sequence is proprietary.

Localization

Early endosome membrane; Peripheral membrane protein; Recycling endosome membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EHD4 Antibody

Western Blot: EHD4 Antibody [NBP2-16287]

Western Blot: EHD4 Antibody [NBP2-16287]

Western Blot: EHD4 Antibody [NBP2-16287] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-16287]

Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-16287]

Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-16287] - Paraffin-embedded rat brain. EHD4 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-16287]

Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-16287]

Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-16287] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.

Applications for EHD4 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EHD4

EHD4 is an EH domain containing protein expressed in the extracellular matrix. EHD4 is the first extracellular matrix protein described that contains an EH domain.

Alternate Names

EH domain containing 4, EH domain-containing protein 4, EH-domain containing 4, HCA10, HCA11, Hepatocellular carcinoma-associated protein 10/11, hepatocellular carcinoma-associated protein HCA11, ortholog of rat pincher, PAST homolog 4, PAST4

Gene Symbol

EHD4

UniProt

Additional EHD4 Products

Product Documents for EHD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EHD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.