Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all EHHADH Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 496-723 of human EHHADH (NP_001957) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

79.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EHHADH Antibody (OTI2C4) - Azide and BSA Free

Western Blot: EHHADH Antibody (OTI2C4)Azide and BSA Free [NBP2-70625]

Western Blot: EHHADH Antibody (OTI2C4)Azide and BSA Free [NBP2-70625]

Western Blot: EHHADH Antibody (OTI2C4) - Azide and BSA Free [NBP2-70625] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EHHADH.
Immunohistochemistry: EHHADH Antibody (OTI2C4) - Azide and BSA Free [NBP2-70625]

Immunohistochemistry: EHHADH Antibody (OTI2C4) - Azide and BSA Free [NBP2-70625]

Immunohistochemistry: EHHADH Antibody (OTI2C4) - Azide and BSA Free [NBP2-70625] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for EHHADH Antibody (OTI2C4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EHHADH

The protein encoded by the EHHADH gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomalbeta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while theC-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomaldisorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for thisgene. (provided by RefSeq)

Alternate Names

enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase, enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase, LBFP, LBP, L-PBE, PBFEMGC120586, peroxisomal, peroxisomal bifunctional enzyme3,2-trans-enoyl-CoA isomerase, peroxisomal enoyl-CoA hydratase

Gene Symbol

EHHADH

Additional EHHADH Products

Product Documents for EHHADH Antibody (OTI2C4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EHHADH Antibody (OTI2C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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