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EI2BL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47475

Novus Biologicals, part of Bio-Techne
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NBP2-47475
NBP2-47475-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HGIPFYVAAPSSSCDLRLETGKEIIIEERPGQELTDVNGVRIAAPGIGVWNPAFDVTPHDLITGGIITELGVFAPEELRTALTTTISSRD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EI2BL Antibody

Western Blot: EI2BL Antibody [NBP2-47475]

Western Blot: EI2BL Antibody [NBP2-47475]

Western Blot: EI2BL Antibody [NBP2-47475] - Analysis in human cell lines HEK293 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/ Immunofluorescence: EI2BL Antibody [NBP2-47475]

Immunocytochemistry/ Immunofluorescence: EI2BL Antibody [NBP2-47475]

Immunocytochemistry/Immunofluorescence: EI2BL Antibody [NBP2-47475] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry: EI2BL Antibody [NBP2-47475]

Immunohistochemistry: EI2BL Antibody [NBP2-47475]

Immunohistochemistry: EI2BL Antibody [NBP2-47475] - Staining of human thyroid gland shows moderate cytoplasmic and nuclear positivity in glandular cells.

Applications for EI2BL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EI2BL

Alternate Names

EC 5.3.1.23, M1Pi, Mediator of RhoA-dependent invasion, methylthioribose-1-phosphate isomerase, methylthioribose-1-phosphate isomerase homolog (S. cerevisiae), MGC3207, MRDI, MTNA, MTR-1-P isomerase, S-methyl-5-thioribose-1-phosphate isomerase, Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein, Ypr118w

Gene Symbol

MRI1

Additional EI2BL Products

Product Documents for EI2BL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EI2BL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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