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eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61573

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-61573

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Porcine

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM298

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A phospho-peptide corresponding to Human eIF2 alpha/EIF2S1 [p Ser51]

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 29449538). Use in Porcine reported in scientific literature (PMID:32184774). This antibody may also react to mouse or rat phospho-eIF2 alpha/EIF2S1 [Ser51].

Modification

p Ser51

Specificity

This antibody reacts to human eIF2 alpha/EIF2S1 only when phosphorylated at Ser51. There is no cross-reactivity to eIF2 alpha/EIF2S1 without phosphorylation at Ser51.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298)

Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) [NBP2-61573] - HeLa cell lysate nontreated (-) or treated (+) with Calyculin A (CalA), using NBP2-61573.
Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) [NBP2-61573] - Human thyroid cancer tissue section using NBP2-61573.
Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) [NBP2-61573] - Human breast cancer tissue section using NBP2-61573.

Applications for eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:100 - 1:200. Use reported in scientific literature (PMID: 29449538).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF2 alpha

eIFs are heterotrimeric initiation complexes that form on the ribosome during protein synthesis. Each eIF serves a distinct function during protein synthesis to help attach, assemble, and drive the ribosome along the mRNA.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Gene Symbol

EIF2S1

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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