EIF2 beta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93284
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Primate (100%), Rabbit (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 283 and 333 of human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa using the numbering given in entry NP_003899.2 (GeneID 8894).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EIF2 beta Antibody
![Western Blot: EIF2 beta Antibody [NB100-93284]](https://resources.bio-techne.com/images/products/EIF2-beta-Antibody-Western-Blot-NB100-93284-img0002.jpg "Western Blot: EIF2 beta Antibody [NB100-93284]")
Western Blot: EIF2 beta Antibody [NB100-93284]
Western Blot: EIF2 beta Antibody [NB100-93284] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-93284 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated less efficiently by NB100-93283, which recognizes an upstream epitope![Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284]](https://resources.bio-techne.com/images/products/EIF2-beta-Antibody-Immunohistochemistry-Paraffin-NB100-93284-img0004.jpg "Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284]")
Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284]
Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2beta/EIF2S2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for EIF2 beta Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EIF2 beta
Alternate Names
DKFZp686L18198, EIF2, EIF2B, EIF2beta, eIF-2-beta, eukaryotic initiation factor 2-beta, eukaryotic translation initiation factor 2 beta, eukaryotic translation initiation factor 2 subunit 2, Eukaryotic translation initiation factor 2 subunit beta, eukaryotic translation initiation factor 2, subunit 2 (beta, 38kD ), eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa, MGC8508
Gene Symbol
EIF2S2
UniProt
Additional EIF2 beta Products
Product Documents for EIF2 beta Antibody
Product Specific Notices for EIF2 beta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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