eIF2A Antibody (S08-8E2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14927

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14927-100ul
NBP3-14927-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-8E2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF2A Antibodies »

Product Summary for eIF2A Antibody (S08-8E2)

Immunogen

A synthetic peptide of human eIF2A (Uniprot#: Q9BY44)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2A Antibody (S08-8E2)

Western Blot: eIF2A Antibody (S08-8E2) [NBP3-14927]

Western Blot: eIF2A Antibody (S08-8E2) [NBP3-14927]

Western Blot: eIF2A Antibody (S08-8E2) [NBP3-14927] - Western blot detection of eIF2A in LnCap, K562, U251MG cell lysates using eIF2A Rabbit mAb (1:500 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.
Immunocytochemistry/ Immunofluorescence: eIF2A Antibody (S08-8E2) [NBP3-14927]

Immunocytochemistry/ Immunofluorescence: eIF2A Antibody (S08-8E2) [NBP3-14927]

Immunocytochemistry/Immunofluorescence: eIF2A Antibody (S08-8E2) [NBP3-14927] - Immunofluorescence of eIF2A (green) in MCF-7 using eIF2A antibody at dilution 1/20, and DAPI (blue)

Applications for eIF2A Antibody (S08-8E2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2A

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Alternate Names

EIF-2, EIF-2A, EIF2AEIF2, eIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )

Gene Symbol

EIF2A

Additional eIF2A Products

Product Documents for eIF2A Antibody (S08-8E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF2A Antibody (S08-8E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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