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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rabbit (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 550 and 600 of human eukaryotic translation initiation factor 2B, subunit 5 epsilon using the numbering given in entry NP_003898.2 (GeneID 8893).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2B epsilon Antibody

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody [NBP1-30449]

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody [NBP1-30449]

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody [NBP1-30449] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: eIF2B epsilon Antibody [NBP1-30449]

Immunoprecipitation: eIF2B epsilon Antibody [NBP1-30449]

Immunoprecipitation: eIF2B epsilon Antibody [NBP1-30449] - Detection of Human eIF2B5 Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF2B5 antibody NBP1-30449 used for IP at 10 mcg/mg lysate. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30448, which recognizes an upstream epitope.

Applications for eIF2B epsilon Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

5-15 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: eIF2B epsilon

The eIF2B epsilon gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other E

Alternate Names

CACH, CLE, EIF-2B, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2BE, EIF2Bepsilon, eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD), eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, LVWM, translation initiation factor eIF-2B subunit epsilon

Gene Symbol

EIF2B5

Additional eIF2B epsilon Products

Product Documents for eIF2B epsilon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF2B epsilon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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