eIF2B3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83986

Novus Biologicals, part of Bio-Techne
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NBP1-83986
NBP1-83986-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF2B3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEANTLNLAPYDACWNACRGDRWEDLSRSQVRCYVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKHLVG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2B3 Antibody

Western Blot: eIF2B3 Antibody [NBP1-83986]

Western Blot: eIF2B3 Antibody [NBP1-83986]

Western Blot: eIF2B3 Antibody [NBP1-83986] - Analysis in control (vector only transfected HEK293T lysate) and EIF2B3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: eIF2B3 Antibody [NBP1-83986]

Immunocytochemistry/ Immunofluorescence: eIF2B3 Antibody [NBP1-83986]

Immunocytochemistry/Immunofluorescence: eIF2B3 Antibody [NBP1-83986] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: eIF2B3 Antibody [NBP1-83986]

Immunohistochemistry-Paraffin: eIF2B3 Antibody [NBP1-83986]

Immunohistochemistry-Paraffin: eIF2B3 Antibody [NBP1-83986] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Applications for eIF2B3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2B3

The protein encoded by the eIF2B3 gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Alternate Names

EIF-2B, eIF-2B GDP-GTP exchange factor subunit gamma, EIF2Bgamma, eukaryotic translation initiation factor 2B, subunit 3 (gamma, 58kD), eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa, translation initiation factor eIF-2B subunit gamma

Gene Symbol

EIF2B3

Additional eIF2B3 Products

Product Documents for eIF2B3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF2B3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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