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EIF2G Antibody (1H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00001968-M03

Novus Biologicals, part of Bio-Techne
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H00001968-M03

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EIF2S3 (NP_001406, 383 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAVKADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EIF2G Antibody (1H3)

Sandwich ELISA: EIF2G Antibody (1H3) [H00001968-M03] - Detection limit for recombinant GST tagged EIF2S3 is 0.3 ng/ml as a capture antibody.

Applications for EIF2G Antibody (1H3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EIF2G

elF-2 gamma (also known as eukaryotic translation initiation factor eIF-2 gamma subunit and eukaryotic translation initiation factor 2 subunit 3) is a 52kD member of the GTP-binding elongation factor family, eIF2G subfamily. This cytoplasmic protein is ubiquitously expressed and has GTPase activity involved in the recruitment of methionyl-tRNA to the 40 S ribosomal subunit. Plays a role in translation initiation and elongation. Heterodimeric interaction with eIF-2 alpha and beta chains, acts as a cofactor for hepatitis C internal ribosome entry site-mediated translation. The Poly6068 antibody recognizes human eIF-2 gamma and has been shown to be useful for Western blotting.

Alternate Names

EIF2, eIF-2gA, EIF2gamma, eIF-2-gamma X, EIF2Geukaryotic translation initiation factor 2, subunit 3 (gamma, 52kD), eIF-2gX, eukaryotic translation initiation factor 2 subunit 3, Eukaryotic translation initiation factor 2 subunit gamma X, eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa, eukaryotic translation initiation factor 2G

Gene Symbol

EIF2S3

Additional EIF2G Products

Product Documents for EIF2G Antibody (1H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EIF2G Antibody (1H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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