EIF3A Antibody (JE58-60)

Western Blot: EIF3A Antibody (JE58-60) [NBP3-32297]

Western Blot: EIF3A Antibody (JE58-60) [NBP3-32297] - Western blot analysis of EIF3A on different lysates with Rabbit anti-EIF3A antibody (NBP3-32297) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: A431 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Raji cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: L6 cell lysate
Lane 8: PC-12 cell lysate

Lysates/proteins at 15 ug/Lane.

Predicted band size: 167 kDa
Observed band size: 167 kDa

Exposure time: 7seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32297) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: EIF3A Antibody (JE58-60) [NBP3-32297]

Immunohistochemistry: EIF3A Antibody (JE58-60) [NBP3-32297] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EIF3A antibody (NBP3-32297) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32297) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: EIF3A Antibody (JE58-60) [NBP3-32297]

Immunocytochemistry/ Immunofluorescence: EIF3A Antibody (JE58-60) [NBP3-32297] - Immunocytochemistry analysis of Hela cells labeling EIF3A with Rabbit anti-EIF3A antibody (NBP3-32297) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EIF3A antibody (NBP3-32297) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.