EIF3A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24570

Novus Biologicals, part of Bio-Techne
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NBP2-24570
NBP2-24570SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Equine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all EIF3A Antibodies »

Product Summary for EIF3A Antibody

Immunogen

A portion of amino acids 550-600 of human eIF3A was used as the immunogen for the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF3A Antibody

Western Blot: EIF3A Antibody [NBP2-24570]

Western Blot: EIF3A Antibody [NBP2-24570]

Western Blot: EIF3A Antibody [NBP2-24570] - analysis of eIF3A in Daudi lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 lysate using eIF3A antibody at 0.5 ug/ml, 0.5 ug/ml and 0.25 ug/ml respectively.
Immunohistochemistry-Paraffin: EIF3A Antibody [NBP2-24570]

Immunohistochemistry-Paraffin: EIF3A Antibody [NBP2-24570]

Immunohistochemistry-Paraffin: EIF3A Antibody [NBP2-24570] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3A antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: EIF3A Antibody [NBP2-24570]

Simple Western: EIF3A Antibody [NBP2-24570]

Simple Western: EIF3A Antibody [NBP2-24570] - Simple Western lane view shows a specific band for EIF3A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for EIF3A Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10 ug/ml

Simple Western

1:50

Western Blot

0.25-1.0 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EIF3A

Eukaryotic initiation factor 3 subunit A (eIF3A) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3A is also known as EIF3S10 (eukaryotic initiation factor 3 subunit 7). eIF3A is the largest subunit of the eIF3 complex. eIF3A expression is regulated through the cell cycle. It's expression peaks in S-phase and it is proposed to play a role in regulating the translation of mRNAs involved in the regulation of cell cycle progression and proliferation. Alternative names for eIF3A include eIF-3-theta, eIF-3 p167, eIF-3 p170, eIF-3 p180, eIF3-p185, EIF3S10, and KIAA0139.

Alternate Names

centrosomin homolog, cytoplasmic protein p167, eIF3 p167, eIF3 p180, eIF3 p185, EIF3, p180 subunit, eIF3a, eIF3-p170, EIF3S10EIF3, eIF-3-theta, eIF3-theta, Eukaryotic translation initiation factor 3 subunit 10, eukaryotic translation initiation factor 3 subunit A, eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD), eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD), eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa, eukaryotic translation initiation factor 3, subunit 10, 170kD, eukaryotic translation initiation factor 3, subunit A, KIAA0139P167, p180, p185, TIF32

Gene Symbol

EIF3A

UniProt

Q14152 (Human)

Additional EIF3A Products

Product Documents for EIF3A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EIF3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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