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EIF3C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46772

Novus Biologicals, part of Bio-Techne
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NBP2-46772

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQARGKKGTDRAAQIELLQLLVQIAAENNLGEGVIVKIKFNIIASLYDYNPNLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF3C Antibody

Western Blot: EIF3C Antibody [NBP2-46772]

Western Blot: EIF3C Antibody [NBP2-46772]

Western Blot: EIF3C Antibody [NBP2-46772] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Analysis of human liver tissue., Lane 6: Human Tonsil tissue.
Immunocytochemistry/ Immunofluorescence: EIF3C Antibody [NBP2-46772]

Immunocytochemistry/ Immunofluorescence: EIF3C Antibody [NBP2-46772]

Immunocytochemistry/Immunofluorescence: EIF3C Antibody [NBP2-46772] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry-Paraffin: EIF3C Antibody [NBP2-46772]

Immunohistochemistry-Paraffin: EIF3C Antibody [NBP2-46772]

Immunohistochemistry-Paraffin: EIF3C Antibody [NBP2-46772] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.

Applications for EIF3C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF3C

eIF3 is the largest of the mammalian translation initiation factors, and consists of at least twelve subunits ranging in mass from 35 to 170 kDa. eIF3 binds to the 40 S ribosome in an early step of translation initiation and promotes the binding of methionyl-tRNAi and mRNA.

Alternate Names

cell migration-inducing protein 17, eIF3 p110, eIF3c, EIF3CL, eIF3-p110, EIF3S8FLJ55450, Eukaryotic translation initiation factor 3 subunit 8, eukaryotic translation initiation factor 3 subunit C, eukaryotic translation initiation factor 3, subunit 8 (110kD), eukaryotic translation initiation factor 3, subunit 8, 110kDa, eukaryotic translation initiation factor 3, subunit C, FLJ53378, FLJ54400, FLJ54404, FLJ55750, FLJ78287, MGC189737, MGC189744

Entrez Gene IDs

8663 (Human)

Gene Symbol

EIF3C

UniProt

Additional EIF3C Products

Product Documents for EIF3C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EIF3C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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