eIF3e Antibody (3H4N2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16184

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16184-100ul
NBP3-16184-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3H4N2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF3e Antibodies »

Product Summary for eIF3e Antibody (3H4N2)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 210-350 of human eIF3e (P60228). QRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF3e Antibody (3H4N2)

Western Blot: eIF3e Antibody (3H4N2) [NBP3-16184]

Western Blot: eIF3e Antibody (3H4N2) [NBP3-16184]

Western Blot: eIF3e Antibody (3H4N2) [NBP3-16184] - Western blot analysis of extracts of various cell lines, using eIF3e Rabbit mAb (NBP3-16184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: eIF3e Antibody (3H4N2) [NBP3-16184]

Immunohistochemistry-Paraffin: eIF3e Antibody (3H4N2) [NBP3-16184]

Immunohistochemistry-Paraffin: eIF3e Antibody (3H4N2) [NBP3-16184] - Immunohistochemistry of paraffin-embedded mouse testis using eIF3e Rabbit mAb (NBP3-16184) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for eIF3e Antibody (3H4N2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF3e

Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stressresponsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

Alternate Names

eIF-3 p48, eIF3e, eIF3-p48, EIF3S6EIF3-P48, Eukaryotic translation initiation factor 3 subunit 6, eukaryotic translation initiation factor 3 subunit E, eukaryotic translation initiation factor 3, subunit 6 (48kD), eukaryotic translation initiation factor 3, subunit 6 48kDa, eukaryotic translation initiation factor 3, subunit E, INT6eIF3-p46, mammary tumor-associated protein INT6, murine mammary tumor integration site 6 (oncogene homolog), Viral integration site protein INT-6 homolog

Gene Symbol

EIF3E

Additional eIF3e Products

Product Documents for eIF3e Antibody (3H4N2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF3e Antibody (3H4N2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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