EIF3F Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16299

Novus Biologicals, part of Bio-Techne
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NBP2-16299
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EIF3F Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3F. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF3F Antibody

Immunocytochemistry/ Immunofluorescence: EIF3F Antibody [NBP2-16299]

Immunocytochemistry/ Immunofluorescence: EIF3F Antibody [NBP2-16299]

Immunocytochemistry/Immunofluorescence: EIF3F Antibody [NBP2-16299] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299]

Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299]

Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - EIF3F antibody detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EIF3F antibody dilution: 1:500.
Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299]

Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299]

Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.
View 1 more images

Applications for EIF3F Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31527668).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EIF3F

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Alternate Names

eIF3 p47, eIF-3-epsilon, eIF3-epsilon, eIF3f, eIF3-p47, EIF3S5eukaryotic translation initiation factor 3 subunit F, Eukaryotic translation initiation factor 3 subunit 5, eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD), eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa, eukaryotic translation initiation factor 3, subunit F

Gene Symbol

EIF3F

UniProt

O00303 (Human)

Additional EIF3F Products

Product Documents for EIF3F Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EIF3F Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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