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eIF3K Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31285

Novus Biologicals, part of Bio-Techne
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NBP1-31285

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rhesus Macaque (100%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for eIF3K Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (88%).

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF3K Antibody

Western Blot: eIF3K Antibody [NBP1-31285]

Western Blot: eIF3K Antibody [NBP1-31285]

Western Blot: eIF3K Antibody [NBP1-31285] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: eIF3K Antibody [NBP1-31285]

Immunocytochemistry/ Immunofluorescence: eIF3K Antibody [NBP1-31285]

Immunocytochemistry/Immunofluorescence: eIF3K Antibody [NBP1-31285] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3K protein stained by EIF3K antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Applications for eIF3K Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF3K

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Alternate Names

ARG134, eIF3k, EIF3-p28, EIF3S12eIF-3 p28, Eukaryotic translation initiation factor 3 subunit 12, eukaryotic translation initiation factor 3 subunit K, eukaryotic translation initiation factor 3, subunit 12, eukaryotic translation initiation factor 3, subunit K, HSPC029, M9, MSTP001, muscle specific, Muscle-specific gene M9 protein, PLAC24, PLAC-24, PLAC-24eIF-3 p25, PRO1474, PTD001

Entrez Gene IDs

27335 (Human); 73830 (Mouse); 515326 (Bovine)

Gene Symbol

EIF3K

UniProt

Additional eIF3K Products

Product Documents for eIF3K Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF3K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.