eIF3X Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93306
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, Immunocytochemistry/ Immunofluorescence, RNA immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1259 and 1309 of human putative eukaryotic translation initiation factor 3 subunit using the numbering given in entry NP_056044.3 (GeneID 23277).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25349259).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for eIF3X Antibody
![Western Blot: eIF3X Antibody [NB100-93306]](https://resources.bio-techne.com/images/products/eIF3X-Antibody-Western-Blot-NB100-93306-img0002.jpg "Western Blot: eIF3X Antibody [NB100-93306]")
Western Blot: eIF3X Antibody [NB100-93306]
Western Blot: eIF3X Antibody [NB100-93306] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibody: Affinity purified rabbit anti-eIF3X antibody NB100-93306 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF3X was also immunoprecipitated by rabbit anti-eIF3X antibody NB100-93305, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3X, the ReliaBLOT (R) Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).Applications for eIF3X Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:32149416).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: eIF3X
Alternate Names
CLU1, DKFZp686L05242, DKFZp686M09233, FLJ23672, hypothetical protein LOC23277, KIAA0664
Entrez Gene IDs
23277 (Human)
Gene Symbol
CLUH
UniProt
Additional eIF3X Products
Product Documents for eIF3X Antibody
Product Specific Notices for eIF3X Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...