eIF4A1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04718

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF4A1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A1 (NP_001407.1). MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF4A1 Antibody - Azide and BSA Free

Western Blot: eIF4A1 AntibodyAzide and BSA Free [NBP3-04718]

Western Blot: eIF4A1 AntibodyAzide and BSA Free [NBP3-04718]

Western Blot: eIF4A1 Antibody [NBP3-04718] - Western blot analysis of extracts of various cell lines, using eIF4A1 antibody (NBP3-04718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [NBP3-04718]

Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [NBP3-04718]

Immunocytochemistry/Immunofluorescence: eIF4A1 Antibody [NBP3-04718] - Immunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb (NBP3-04718) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [NBP3-04718]

Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [NBP3-04718]

Immunocytochemistry/Immunofluorescence: eIF4A1 Antibody [NBP3-04718] - Immunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb (NBP3-04718) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for eIF4A1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF4A1

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-1, DDX2Aeukaryotic initiation factor 4A-I, EC 3.6.1, EC 3.6.4.13, EIF4A, EIF-4A, eIF-4A-I, eIF4A-I, eukaryotic initiation factor 4AI, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A, isoform 1, eukaryotic translation initiation factor 4A1

Gene Symbol

EIF4A1

Additional eIF4A1 Products

Product Documents for eIF4A1 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF4A1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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