eIF4E Antibody (7A7P2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15629

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15629-100ul
NBP3-15629-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7A7P2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF4E Antibodies »

Product Summary for eIF4E Antibody (7A7P2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 118-217 of human eIF4E (P06730). NKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4E Antibody (7A7P2)

Western Blot: eIF4E Antibody (7A7P2) [NBP3-15629]

Western Blot: eIF4E Antibody (7A7P2) [NBP3-15629]

Western Blot: eIF4E Antibody (7A7P2) [NBP3-15629] - Western blot analysis of extracts of various cell lines, using eIF4E antibody (NBP3-15629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunoprecipitation: eIF4E Antibody (7A7P2) [NBP3-15629]

Immunoprecipitation: eIF4E Antibody (7A7P2) [NBP3-15629]

Immunoprecipitation: eIF4E Antibody (7A7P2) [NBP3-15629] - Immunoprecipitation analysis of 600ug extracts of Mouse testis using 3ug eIF4E antibody (NBP3-15629). Western blot was performed from the immunoprecipitate using eIF4E (NBP3-15629) at a dilition of 1:500.

Applications for eIF4E Antibody (7A7P2)

Application
Recommended Usage

Immunoprecipitation

0.5μg-4μg antibody for 400μg-600μg extracts of whole cells

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF4E

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

Additional eIF4E Products

Product Documents for eIF4E Antibody (7A7P2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF4E Antibody (7A7P2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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