eIF4E [p Ser209] Antibody (SR1401)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21524

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21524-100ul
NBP3-21524-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1401

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF4E Antibodies »

Product Summary for eIF4E [p Ser209] Antibody (SR1401)

Immunogen

A synthesized peptide derived from human phospho-eIF4E [Ser209] (Uniprot #: P06730 )

Modification

p Ser209

Specificity

Detects endogenous levels of total phospho-eIF4E [Ser209]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4E [p Ser209] Antibody (SR1401)

Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] -

Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21524.

Western Blot: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] -

Western Blot: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Western blot analysis using NBP3-21524 in (1) HEK293 cell lysate; (2) Mouse spleen lysate.

Applications for eIF4E [p Ser209] Antibody (SR1401)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4E

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

Additional eIF4E Products

Product Documents for eIF4E [p Ser209] Antibody (SR1401)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF4E [p Ser209] Antibody (SR1401)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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