eIF4GII Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16309

Novus Biologicals, part of Bio-Techne
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NBP2-16309
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF4GII Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF4GII. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

177 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4GII Antibody

Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with eIF4GII antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - eIF4GII antibody detects eIF4GII protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. eIF4GII stained by eIF4GII antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309]

Western Blot: eIF4GII Antibody [NBP2-16309] - A: Marker. B: mouse testes. 6% SDS-PAGE gel, antibody diluted 1:10000. This image was submitted via customer review.
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Applications for eIF4GII Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Reviewed Applications

Read 1 review rated 5 using NBP2-16309 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF4GII

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1

Alternate Names

eIF-4G 3, eIF-4-gamma 3, eIF-4-gamma II, eIF4GIIeIF4G 3, eukaryotic translation initiation factor 4 gamma 3, eukaryotic translation initiation factor 4 gamma, 3

Gene Symbol

EIF4G3

UniProt

O43432 (Human)

Additional eIF4GII Products

Product Documents for eIF4GII Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF4GII Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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