eIF4H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99470

Novus Biologicals, part of Bio-Techne
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NBP2-99470-100ul
NBP2-99470-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF4H Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human eIF4H.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for eIF4H Antibody

Western Blot: eIF4H Antibody [NBP2-99470]

Western Blot: eIF4H Antibody [NBP2-99470]

Western Blot: eIF4H Antibody [NBP2-99470] - Anti-eIF4H rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa
Immunocytochemistry/ Immunofluorescence: eIF4H Antibody [NBP2-99470]

Immunocytochemistry/ Immunofluorescence: eIF4H Antibody [NBP2-99470]

Immunocytochemistry/Immunofluorescence: eIF4H Antibody [NBP2-99470] - Immunofluorescence staining of eIF4H in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human eIF4H polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for eIF4H Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4H

eIF4H encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Alternate Names

eukaryotic translation initiation factor 4H, KIAA0038eIF-4H, WBSCR1, Williams-Beuren syndrome chromosome region 1, WSCR1Williams-Beuren syndrome chromosomal region 1 protein

Gene Symbol

EIF4H

Additional eIF4H Products

Product Documents for eIF4H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF4H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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